Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Capsaicin Enhanced the Efficacy of Photodynamic Therapy Against Osteosarcoma via a Pro-Death Strategy by Inducing Ferroptosis and Alleviating Hypoxia.

doi: 10.1002/smll.202306916

Figure Lengend Snippet: Figure 3. Responsive activation of TRPV1-mediated intracellular Ca2+ concentration. A) CLSM images of 143B and HOS cells in various groups after using the Fluo-4 probe to monitor Ca2+ influx. The scale bar is 20 μm. B,C) FC quantitative value of Ca2+ uptake in 143B and HOS cells after different treatments. D) The protein expression level of TRPV1 was determined by western blot analysis. E) Statistical analyses of TRPV1 (the data are presented as the mean ± SD, n = 3, ***p < 0.001).

Article Snippet: Mouse anti-human TRPV1 (cat. 66983-1-Ig), mouse anti-human NQO1 (cat. 67240-1-Ig), rabbit anti-human GCLM (cat. 14241-1-AP), rabbit anti-human PCNA (cat. 10205-2-AP), rabbit anti-human KI67 (cat. 27309-1-AP), and rabbit antihuman HIF-1α (cat. 20960-1-AP) were acquired from Proteintech Corp (USA).

Techniques: Activation Assay, Concentration Assay, Expressing, Western Blot

Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Capsaicin Enhanced the Efficacy of Photodynamic Therapy Against Osteosarcoma via a Pro-Death Strategy by Inducing Ferroptosis and Alleviating Hypoxia.

doi: 10.1002/smll.202306916

Figure Lengend Snippet: Figure 4. Evaluation of ferroptosis. The production of Lipid-ROS in A) 143B and B) HOS cells after different interventions as measured by CLSM. The scale bars represent 20 μm. C) FC analysis of Lipid-ROS after different interventions in 143B and HOS cells. D,E) Confocal images and FC analyses of ROS generation in 143B and HOS cells treated with different treatments and stained with DCFH-DA (scale bar = 20 μm). F,G) Quantitative value of intracellular C11-BODIPY and DCFH-DA FL intensity (the data are presented as the mean ± SD, n = 3, ***p < 0.001). H) The morphology of mitochondria after various treatments as observed by TEM. The scale bars represent 2 μm. I) The expression level of Gpx4 protein was measured by western blot analysis. J) The expression of TRPV1 and Gpx4 by IF observation after different treatments in 143B cells (scale bar = 100 μm).

Article Snippet: Mouse anti-human TRPV1 (cat. 66983-1-Ig), mouse anti-human NQO1 (cat. 67240-1-Ig), rabbit anti-human GCLM (cat. 14241-1-AP), rabbit anti-human PCNA (cat. 10205-2-AP), rabbit anti-human KI67 (cat. 27309-1-AP), and rabbit antihuman HIF-1α (cat. 20960-1-AP) were acquired from Proteintech Corp (USA).

Techniques: Staining, Expressing, Western Blot

Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Capsaicin Enhanced the Efficacy of Photodynamic Therapy Against Osteosarcoma via a Pro-Death Strategy by Inducing Ferroptosis and Alleviating Hypoxia.

doi: 10.1002/smll.202306916

Figure Lengend Snippet: Figure 5. A) In MG63, HOS, and 143B cells, the effects of CAP on the intracellular oxygen level after different treatments were observed and analyzed by confocal microscopy (scale bar = 20 μm) and FC. B) Quantitative value of intracellular Image-iT Green Hypoxia Reagent FL intensity (the data are presented as the mean ± SD, n = 3, ***p < 0.001). C,D) The expression level of HIF-1𝛼protein was analyzed by western blot (the data are presented as the mean ± SD, n = 3, ***p < 0.001). E,F) The expression of TRPV1 and HIF-1𝛼by IF observation after different treatments in 143B and HOS cells (scale bar = 100 μm).

Article Snippet: Mouse anti-human TRPV1 (cat. 66983-1-Ig), mouse anti-human NQO1 (cat. 67240-1-Ig), rabbit anti-human GCLM (cat. 14241-1-AP), rabbit anti-human PCNA (cat. 10205-2-AP), rabbit anti-human KI67 (cat. 27309-1-AP), and rabbit antihuman HIF-1α (cat. 20960-1-AP) were acquired from Proteintech Corp (USA).

Techniques: Confocal Microscopy, Expressing, Western Blot

Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Capsaicin Enhanced the Efficacy of Photodynamic Therapy Against Osteosarcoma via a Pro-Death Strategy by Inducing Ferroptosis and Alleviating Hypoxia.

doi: 10.1002/smll.202306916

Figure Lengend Snippet: Figure 8. Evaluation and biosafety of different treatments. A) H&E staining and images of PCNA, Ki67, TRPV1, Gpx4, and HIF-1𝛼. IHC staining in tumor specimens after various treatments (scale bar = 100 μm). B–F) IOD values in various groups were analyzed by processing IHC staining through ImagePro Plus. G) Triple IF staining of TRPV1 (red), Gpx4 (orange), and HIF-1𝛼(green) in tumor specimens after various treatments (scale bar = 50 μm). H) TUNEL staining of tumor sections after various treatments (scale bar = 50 μm). I) H&E staining of major organs of mice after different treatments (scale bar = 100 μm). J) Hematological and blood biochemical tests of mice after various treatments.

Article Snippet: Mouse anti-human TRPV1 (cat. 66983-1-Ig), mouse anti-human NQO1 (cat. 67240-1-Ig), rabbit anti-human GCLM (cat. 14241-1-AP), rabbit anti-human PCNA (cat. 10205-2-AP), rabbit anti-human KI67 (cat. 27309-1-AP), and rabbit antihuman HIF-1α (cat. 20960-1-AP) were acquired from Proteintech Corp (USA).

Techniques: Staining, Immunohistochemistry, TUNEL Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: Comparative graph showing the hind paw withdrawal threshold and latency. Black: control group, red: ICS group, blue: ICS + electroacupuncture (EA) group, green: transient receptor vanilloid member one deletion ( Trpv1 −/− ) group. ∗ indicates statistical significance with p < 0.05 when compared with the control group. # indicates statistical significance with p < 0.05 when compared with the ICS group. (a) Mechanical hyperalgesia measured by the von Frey test in the four groups. (b) Thermal hyperalgesia assessed by the Hargreaves test in the four groups. (c) Schematic illustration showing the ICS procedure. Control mice stayed in a 24°C environment day and night for five days. Mice subjected to ICS were kept in a 4°C environment for 17.5 hours (from 4:30 p.m. to 10:00 a.m.) from Day 0 to 3. Between 10:00 a.m. and 4:30 p.m. (total duration: 6.5 hours), they were placed in a room at 24°C. In this 6.5-hour period, the mice were subjected to intermittent environment temperature change (24°C and 4°C) for 30 min each time. The procedure was terminated on Day 3 at 10:00 a.m., and fibromyalgia-like pain was assessed.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: (a) Expression of extracellular neurotransmitters ((A) HMGB1, (B) S100B), receptors ((C) RAGE, (D) TLR2, (E) TLR4), and cytoplasmic inflammation signal molecules ((F) pPI3K, (G) pAkt, (H) pmTOR, (I) pERK, (J) pp38, (K) pJNK, (L) pNF- κ B) in the mouse PFC. The four lanes on the immunoblots correspond to the protein bands of, in order, control (Con), ICS-induced fibromyalgia (ICS), electroacupuncture (EA), and Trpv1 −/− groups. ∗ indicates statistical significance with P < 0.05 when compared with the Con group. # indicates statistical significance with P < 0.05 when compared with the ICS group. (b) Immunofluorescence staining of TLR2 and pNF- κ B in the mouse PFC. Immunopositive signals (light green, indicated by a red arrow) were detected for TLR2 and pNF- κ B. Scale bar: 100 μ m.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Expressing, Western Blot, Control, Immunofluorescence, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: (a) Expression of extracellular neurotransmitters ((A) HMGB1, (B) S100B), receptors ((C) RAGE, (D) TLR2, (E) TLR4), and cytoplasmic inflammation signal molecules ((F) pPI3K, (G) pAkt, (H) pmTOR, (I) pERK, (J) pp38, (K) pJNK, (L) pNF- κ B) in the mouse SSC. The four lanes on the immunoblots correspond to the protein bands of, in order, control (Con), ICS-induced fibromyalgia (ICS), electroacupuncture (EA), and Trpv1 −/− groups. ∗ indicates statistical significance with P < 0.05 when compared with the Con group. # indicates P < 0.05 statistical significance when compared with the ICS group. (b) Immunofluorescence staining of TLR2 and pNF- κ B in the mouse SSC. Immunopositive signals (light green, indicated by a red arrow) were detected for TLR2 and pNF- κ B. Scale bar: 100 μ m.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Expressing, Western Blot, Control, Immunofluorescence, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: (a) Expression of extracellular neurotransmitters (A) HMGB1, (B) S100B), receptors (C) RAGE, (D) TLR2, (E) TLR4) and cytoplasmic inflammation signal molecules (F) pPI3K, (G) pAkt, (H) pmTOR, (I) pERK, (J) pp38, (K) pJNK, (L) pNF- κ B) in the mouse thalamus. The four lanes on the immunoblots correspond to the protein bands of, in order, control (Con), ICS-induced fibromyalgia (ICS), electroacupuncture (EA) and Trpv1 −/− groups. ∗ indicates statistical significance with P < 0.05 when compared with the Con group. # indicates statistical significance with P < 0.05 when compared to the ICS group. (b) Immunofluorescence staining of TLR2 and pNF- κ B in the mouse thalamus. Immunopositive signals (light green, indicated by a red arrow) were detected for TLR2 and pNF- κ B. Scale bar: 100 μ m.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Expressing, Western Blot, Control, Immunofluorescence, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: (a) The expression of extracellular neurotransmitters ((A) HMGB1, (B) S100B), receptors ((C) RAGE, (D) TLR2, (E) TLR4), and cytoplasmic inflammation signal molecules ((F) pPI3K, (G) pAkt, (H) pmTOR, (I) pERK, (J) pp38, (K) pJNK, (L) pNF- κ B) in the mouse amygdala. The four lanes on the immunoblots correspond to the protein bands of, in order, control (Con), ICS-induced fibromyalgia (ICS), electroacupuncture (EA), and Trpv1 −/− groups. ∗ indicates statistical significance with P < 0.05 when compared with the Con group. # indicates statistical significance with P < 0.05 when compared with the ICS group. (b) Immunofluorescence staining of TLR2 and pNF- κ B in the mouse amygdala. Immunopositive signals (light green, indicated by a red arrow) were detected for TLR2 and pNF- κ B. Scale bar: 100 μ m.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Expressing, Western Blot, Control, Immunofluorescence, Staining

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Electroacupuncture Reduces Fibromyalgia Pain by Attenuating the HMGB1, S100B, and TRPV1 Signalling Pathways in the Mouse Brain

doi: 10.1155/2022/2242074

Figure Lengend Snippet: Schematic illustration of neuronal and non-neuronal mechanisms underlying EA-mediated analgesic effect on ICS-induced fibromyalgia pain. The summary diagram shows the importance of and mechanisms involving glial cells (astrocytes and microglia) and TRPV1 in fibromyalgia pain. EA inhibits HMGB1 and S100B release from non-neuronal cells or directly inhibits TRPV1 on the plasma membrane. Mice with a TRPV1 gene deletion ( Trpv1 −/− ) have the same phenotype than mice treated with EA.

Article Snippet: The membrane was blocked with 5% nonfat milk in TBS-T buffer (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.1% Tween-20) and then incubated with a primary antibody against TRPV1 (∼95 kDa, 1 : 1000; Alomone, Israel), HMGB1 (∼28 kDa, 1 : 1000; Alomone, Israel), S100B (∼10 kDa, 1 : 1000; Millipore, USA), TLR4 (∼35 kDa, 1 : 1000; Millipore, USA), RAGE (∼42 kDa, 1 : 1000; Millipore, USA), pPI3K (∼125 kDa, 1 : 1000; Millipore, USA), pAkt (∼60 kDa, 1 : 1000; Millipore, USA), pmTOR (∼60 kDa, 1 : 500; Millipore, USA), pERK1/2 (∼42–44 kDa, 1 : 1000; Millipore, USA), pp38 (∼42 kDa, 1 : 1000; Millipore, USA), pJNK (∼42 kDa, 1 : 1000; Millipore, USA), and pNF- κ B (∼65 kDa, 1 : 1000; Millipore, USA) in TBS-T with 1% bovine serum albumin.

Techniques: Clinical Proteomics, Membrane

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: TRPV1 activation in human Langerhans cells and T cells inhibits mucosal HIV-1 infection via CGRP-dependent and independent mechanisms.

doi: 10.1073/pnas.2302509120

Figure Lengend Snippet: Fig. 2. LCs express functional TRPV1 and CLR. (A and B) Inner foreskin epidermal cell suspensions (A) and MDLCs (B) were stained for surface expression of TRPV1 and CLR, and examined by flow cytometry. Representative overlays show TRPV1 and CLR expression (grey histograms) vs. matched isotype controls (line histograms), in epidermal cells and MDLCs gated as show in Fig. 1. Numbers represent mean ± SEM percentages of positive cells (derived from n = 3 to 6 experiments, using tissues/cells from different donors). (C) Relative TRPV1 mRNA expression in MDLCs from three different human individuals (1 to 3) normalized to beta actin, with total human brain (HB) mRNA serving as positive control. (D) MDLCs were loaded with the Ca2+ indicator Indo-1 and examined over time by flow cytometry. Representative graphs (n = 3) show the normalized bound/free Ca2+ ratio, indicative of Ca2+ influx, at baseline (t = 0 s, set to 1) and following treatment (t = 60 s, arrow) with the indicated concentrations of CP (Left) or RTX (Right). Ionomycin (1 μM; broken lines) served as positive control.

Article Snippet: 120 No. 22 e2302509120 https://doi.org/10.1073/pnas.2302509120 7 of 8 CCR5 (clone 2D7, 1:10; Pharmingen) or CD169 (clone 7-239, 1:20; Miltenyi); FITC–conjugated rabbit–antihuman TRPV1 or CLR (1:25; Alomone Labs).

Techniques: Functional Assay, Staining, Expressing, Flow Cytometry, Derivative Assay, Positive Control

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: TRPV1 activation in human Langerhans cells and T cells inhibits mucosal HIV-1 infection via CGRP-dependent and independent mechanisms.

doi: 10.1073/pnas.2302509120

Figure Lengend Snippet: Fig. 3. TRPV1 activation in MDLCs and CD4+ T cells inhibits HIV-1 transfer and infection in vitro. (A, B, D, and E) MDLCs were treated for 24 h with the indicated molar concentrations of CP or CGRP. The TRPV1 antagonist A425619 (B) and the CGRP receptor antagonist BIBN4096 (D) were added 15 min before addition of CP or CGRP. The cells were then washed, pulsed with HIV-1 for 4 h, washed again, and cocultured with autologous CD4+ T cells. In some experiments, a CCL3 neutralizing Ab was added during the co-culture period (E). HIV-1 replication was evaluated a week later by measuring p24 content in the co-culture supernatants using ELISA. Shown are mean ± SEM (n = 3 to 4) percentages of HIV-1 transfer, normalized against untreated cells serving as the 100% set point. (C) MDLCs were treated for 24 h with the indicated molar concentrations of CP, Rut or CGRP. Culture supernatants were collected immediately after CP and Rut treatment, or following extensive washing and culture in fresh medium for additional 24 h after CGRP treatment, and an EIA was used to measure CGRP levels. Shown are mean ± SEM (n = 3) levels of secreted CGRP per 106 MDLCs. (F) MDLCs were treated for 24 h with CP or CGRP, washed and pulsed with HIV-1 for 2 h. The cells were next washed again, treated immediately or 72 h later with trypsin for 10 min to remove surface-bound virus, lysed, and intracellular HIV-1 was measured in the cell lysates using p24 ELISA. Shown are mean ± SEM (n = 4) concentrations of intracellular HIV-1 p24. (G) PHA/IL-2-activated blood primary CD4+ T cells were treated for 24 h with the indicated molar concentrations of CP or CGRP. The cells were then washed, pulsed with HIV-1 for 4 h, washed again, and HIV- 1 replication was evaluated 3 d later by p24 intracellular staining and flow cytometry. Results (n = 5 to 8) are shown as in A. In all graphs, *P < 0.0500, **P < 0.0050, ***P < 0.0005, Student’s t-test.

Article Snippet: 120 No. 22 e2302509120 https://doi.org/10.1073/pnas.2302509120 7 of 8 CCR5 (clone 2D7, 1:10; Pharmingen) or CD169 (clone 7-239, 1:20; Miltenyi); FITC–conjugated rabbit–antihuman TRPV1 or CLR (1:25; Alomone Labs).

Techniques: Activation Assay, Infection, In Vitro, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Virus, Staining, Flow Cytometry

Journal: Investigative and Clinical Urology

Article Title: Changes in transient receptor potential vanilloid 1 and transient receptor potential vanilloid 4 in patients with lower urinary tract dysfunction

doi: 10.4111/icu.20210418

Figure Lengend Snippet: Comparisons of TRPV1 and TRPV4 expression levels (A) between the DU group and non-DU group and (B) between the urgency group and non-urgency group. TRPV, transient receptor potential vanilloid; DU, detrusor underactivity. * Significant difference between the two groups (p<0.05).

Article Snippet: After two freeze-thaw cycles were performed, the homogenate was centrifuged at 2°C to 8°C for 5 minutes at 5,000× g . The levels of TRPV1 and TRPV4 in the specimen were measured according to the manufacturer’s instructions using a human TRPV1 ELISA kit and a human TRPV4 ELISA kit (Cusabio Biotech Co., Houston, TX, USA).

Techniques: Expressing

Journal: Investigative and Clinical Urology

Article Title: Changes in transient receptor potential vanilloid 1 and transient receptor potential vanilloid 4 in patients with lower urinary tract dysfunction

doi: 10.4111/icu.20210418

Figure Lengend Snippet: Correlation of TRPV1 and TRPV4 with urodynamic parameters

Article Snippet: After two freeze-thaw cycles were performed, the homogenate was centrifuged at 2°C to 8°C for 5 minutes at 5,000× g . The levels of TRPV1 and TRPV4 in the specimen were measured according to the manufacturer’s instructions using a human TRPV1 ELISA kit and a human TRPV4 ELISA kit (Cusabio Biotech Co., Houston, TX, USA).

Techniques:

Journal: Investigative and Clinical Urology

Article Title: Changes in transient receptor potential vanilloid 1 and transient receptor potential vanilloid 4 in patients with lower urinary tract dysfunction

doi: 10.4111/icu.20210418

Figure Lengend Snippet: Immunofluorescence staining of TRPV1 and TRPV4. (A) TRPV1 in the DU group. (B) TRPV1 in the non-DU group. (C) TRPV4 in the DU group. (D) TRPV4 in the non-DU group. (E) TRPV1 in the urgency group. (F) TRPV1 in the non-urgency group. (G) TRPV4 in the urgency group. (H) TRPV4 in the non-urgency group. Scale bars: 200 µm. TRPV, transient receptor potential vanilloid; DU, detrusor underactivity.

Article Snippet: After two freeze-thaw cycles were performed, the homogenate was centrifuged at 2°C to 8°C for 5 minutes at 5,000× g . The levels of TRPV1 and TRPV4 in the specimen were measured according to the manufacturer’s instructions using a human TRPV1 ELISA kit and a human TRPV4 ELISA kit (Cusabio Biotech Co., Houston, TX, USA).

Techniques: Immunofluorescence, Staining